isotype matched control antibody (R&D Systems)
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Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/igg2b+isotype+control+antibody/Mouse+IgG2B+APC-conjugated+Antibody/us12613244-531-21-26
Average 93 stars, based on 103 article reviews
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Control:Article Title: Synthesis and Biological Evaluation of Herceptin-Conjugated Liposomes Loaded with Lipocalin-2 siRNA for the Treatment of Inflammatory Breast Cancer Article Snippet: The cells were then incubated for 30 min with a primary antibody against human ErbB2/HER2 (R&D Systems, Minneapolis, MN, USA) (catalog: MAB1129) at 0.25 μg of antibody per million cells. .. An Article Title: Early Secretory Antigenic Target-6 Drives Matrix Metalloproteinase-10 Gene Expression and Secretion in Tuberculosis Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and horse radish peroxidase-conjugated anti-rabbit IgG (Cell Signaling, Boston, MA) antibodies were used for Western blot. .. MMP-10–neutralizing antibody and Article Title: ESAT-6 Drives MMP-10 Gene Expression and Secretion in Tuberculosis Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and HRP-conjugated anti-rabbit IgG (Cell Signaling, Boston, USA) antibodies were used for western blot. .. MMP10 neutralizing antibody and Article Title: Biomimetic lipid nanoparticles for RNA delivery to breast cancer microenvironment cells by enhanced homotypic and heterotypic adhesion. Article Snippet: • Biomimetic lipid nanoparticles camouflaged with cancer cell membrane nanoghosts were developed for targeted Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or Article Title: Role of porcine reproductive and respiratory syndrome virus nucleocapsid protein in induction of interleukin-10 and regulatory T-lymphocytes (Treg). Article Snippet: For neutralization of porcine IL-10 bioactivity, the anti-porcine IL-10 mAb (clone #148801, R&D Systems) was added into the MoDC culture system at the time of infection with PRRSV (before), when autologous PBL was added (after), or at both steps (both) at 5 mg ml21. .. In the control system, anti-IL-10 mAb was replaced with the Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells. Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti- MUC1- C (HM- 1630- P1ABX, 1:100 dilution; Thermo Fisher Scientific), antiMICA (ab150355, 1:1000 dilution; Abcam), anti- MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; SigmaAldrich), anti- CD9 (13 174S, 1:1000 dilution; CST), antiCD63 (ab59479, 1:1000 dilution; Abcam), anti- CD81 (56 039S, 1:1000 dilution; CST), anti- ERp5 (1:2500; 18 233–1- AP, Proteintech, Rosemont, Illinois, USA) and anti- RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or Negative Control:Article Title: Synthesis and Biological Evaluation of Herceptin-Conjugated Liposomes Loaded with Lipocalin-2 siRNA for the Treatment of Inflammatory Breast Cancer Article Snippet: The cells were then incubated for 30 min with a primary antibody against human ErbB2/HER2 (R&D Systems, Minneapolis, MN, USA) (catalog: MAB1129) at 0.25 μg of antibody per million cells. .. An Functional Assay:Article Title: Early Secretory Antigenic Target-6 Drives Matrix Metalloproteinase-10 Gene Expression and Secretion in Tuberculosis Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and horse radish peroxidase-conjugated anti-rabbit IgG (Cell Signaling, Boston, MA) antibodies were used for Western blot. .. MMP-10–neutralizing antibody and Article Title: ESAT-6 Drives MMP-10 Gene Expression and Secretion in Tuberculosis Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and HRP-conjugated anti-rabbit IgG (Cell Signaling, Boston, USA) antibodies were used for western blot. .. MMP10 neutralizing antibody and Saline:Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells. Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti- MUC1- C (HM- 1630- P1ABX, 1:100 dilution; Thermo Fisher Scientific), antiMICA (ab150355, 1:1000 dilution; Abcam), anti- MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; SigmaAldrich), anti- CD9 (13 174S, 1:1000 dilution; CST), antiCD63 (ab59479, 1:1000 dilution; Abcam), anti- CD81 (56 039S, 1:1000 dilution; CST), anti- ERp5 (1:2500; 18 233–1- AP, Proteintech, Rosemont, Illinois, USA) and anti- RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or Incubation:Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells. Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti- MUC1- C (HM- 1630- P1ABX, 1:100 dilution; Thermo Fisher Scientific), antiMICA (ab150355, 1:1000 dilution; Abcam), anti- MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; SigmaAldrich), anti- CD9 (13 174S, 1:1000 dilution; CST), antiCD63 (ab59479, 1:1000 dilution; Abcam), anti- CD81 (56 039S, 1:1000 dilution; CST), anti- ERp5 (1:2500; 18 233–1- AP, Proteintech, Rosemont, Illinois, USA) and anti- RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or |
