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isotype matched control antibody  (R&D Systems)


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    R&D Systems isotype matched control antibody
    Isotype Matched Control Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igg2b+isotype+control+antibody/Mouse+IgG2B+APC-conjugated+Antibody/us12613244-531-21-26
    Average 93 stars, based on 103 article reviews
    isotype matched control antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Control:

    Article Title: Synthesis and Biological Evaluation of Herceptin-Conjugated Liposomes Loaded with Lipocalin-2 siRNA for the Treatment of Inflammatory Breast Cancer
    Article Snippet: The cells were then incubated for 30 min with a primary antibody against human ErbB2/HER2 (R&D Systems, Minneapolis, MN, USA) (catalog: MAB1129) at 0.25 μg of antibody per million cells. .. An IgG2B isotype control antibody (R&D Systems cat. MAB0041) was used as a negative control. .. After this period, the cells were centrifuged and incubated for 10 min at room temperature with phycoerythrin anti-mouse IgG secondary antibody (R&D Systems cat. F0102B), diluted 1:200 in 1% BSA/PBS, and centrifuged again.

    Article Title: Early Secretory Antigenic Target-6 Drives Matrix Metalloproteinase-10 Gene Expression and Secretion in Tuberculosis
    Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and horse radish peroxidase-conjugated anti-rabbit IgG (Cell Signaling, Boston, MA) antibodies were used for Western blot. .. MMP-10–neutralizing antibody and IgG2B isotype control antibody (R&D Systems, Abingdon, UK) were used for functional assays. .. Lipoarabinomannan (LAM; Colorado State University, Fort Collins, CO) and peptides covering the entire ESAT-6 sequence (Pepceuticals, Enderby, UK) were used to stimulate macrophages.

    Article Title: ESAT-6 Drives MMP-10 Gene Expression and Secretion in Tuberculosis
    Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and HRP-conjugated anti-rabbit IgG (Cell Signaling, Boston, USA) antibodies were used for western blot. .. MMP10 neutralizing antibody and IgG2B isotype control antibody (R&D Systems, Abingdon, UK) were used for functional assays. .. Lipoarabinomannan (LAM, Colorado State University, USA) and peptides covering the entire ESAT-6 sequence (Pepceuticals, Enderby, UK) were used to stimulate macrophages.

    Article Title: Biomimetic lipid nanoparticles for RNA delivery to breast cancer microenvironment cells by enhanced homotypic and heterotypic adhesion.
    Article Snippet: • Biomimetic lipid nanoparticles camouflaged with cancer cell membrane nanoghosts were developed for targeted

    Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells
    Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or IgG2B isotype control antibody (IC0041G, R&D systems) and (2) anti-HLA-E (342603; BioLegend, San Diego, California, USA), anti-HLA-ABC (555552; BD Biosciences, San Jose, California, USA) or an IgG1 isotype control antibody (555748; BD Biosciences) for 30 min on ice. ..

    Article Title: Role of porcine reproductive and respiratory syndrome virus nucleocapsid protein in induction of interleukin-10 and regulatory T-lymphocytes (Treg).
    Article Snippet: For neutralization of porcine IL-10 bioactivity, the anti-porcine IL-10 mAb (clone #148801, R&D Systems) was added into the MoDC culture system at the time of infection with PRRSV (before), when autologous PBL was added (after), or at both steps (both) at 5 mg ml21. .. In the control system, anti-IL-10 mAb was replaced with the IgG2b isotype-control antibody (clone #20116, R&D Systems). ..

    Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells.
    Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti- MUC1- C (HM- 1630- P1ABX, 1:100 dilution; Thermo Fisher Scientific), antiMICA (ab150355, 1:1000 dilution; Abcam), anti- MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; SigmaAldrich), anti- CD9 (13 174S, 1:1000 dilution; CST), antiCD63 (ab59479, 1:1000 dilution; Abcam), anti- CD81 (56 039S, 1:1000 dilution; CST), anti- ERp5 (1:2500; 18 233–1- AP, Proteintech, Rosemont, Illinois, USA) and anti- RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or IgG2B isotype control antibody (IC0041G, R&D systems) and (2) anti- HLA- E (342603; BioLegend, San Diego, California, USA), antiHLA- ABC (555552; BD Biosciences, San Jose, California, USA) or an IgG1 isotype control antibody (555748; BD Biosciences) for 30 min on ice. ..

    Negative Control:

    Article Title: Synthesis and Biological Evaluation of Herceptin-Conjugated Liposomes Loaded with Lipocalin-2 siRNA for the Treatment of Inflammatory Breast Cancer
    Article Snippet: The cells were then incubated for 30 min with a primary antibody against human ErbB2/HER2 (R&D Systems, Minneapolis, MN, USA) (catalog: MAB1129) at 0.25 μg of antibody per million cells. .. An IgG2B isotype control antibody (R&D Systems cat. MAB0041) was used as a negative control. .. After this period, the cells were centrifuged and incubated for 10 min at room temperature with phycoerythrin anti-mouse IgG secondary antibody (R&D Systems cat. F0102B), diluted 1:200 in 1% BSA/PBS, and centrifuged again.

    Functional Assay:

    Article Title: Early Secretory Antigenic Target-6 Drives Matrix Metalloproteinase-10 Gene Expression and Secretion in Tuberculosis
    Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and horse radish peroxidase-conjugated anti-rabbit IgG (Cell Signaling, Boston, MA) antibodies were used for Western blot. .. MMP-10–neutralizing antibody and IgG2B isotype control antibody (R&D Systems, Abingdon, UK) were used for functional assays. .. Lipoarabinomannan (LAM; Colorado State University, Fort Collins, CO) and peptides covering the entire ESAT-6 sequence (Pepceuticals, Enderby, UK) were used to stimulate macrophages.

    Article Title: ESAT-6 Drives MMP-10 Gene Expression and Secretion in Tuberculosis
    Article Snippet: Anti-human MMP-10 (Abcam, Cambridge, UK) and HRP-conjugated anti-rabbit IgG (Cell Signaling, Boston, USA) antibodies were used for western blot. .. MMP10 neutralizing antibody and IgG2B isotype control antibody (R&D Systems, Abingdon, UK) were used for functional assays. .. Lipoarabinomannan (LAM, Colorado State University, USA) and peptides covering the entire ESAT-6 sequence (Pepceuticals, Enderby, UK) were used to stimulate macrophages.

    Saline:

    Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells
    Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or IgG2B isotype control antibody (IC0041G, R&D systems) and (2) anti-HLA-E (342603; BioLegend, San Diego, California, USA), anti-HLA-ABC (555552; BD Biosciences, San Jose, California, USA) or an IgG1 isotype control antibody (555748; BD Biosciences) for 30 min on ice. ..

    Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells.
    Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti- MUC1- C (HM- 1630- P1ABX, 1:100 dilution; Thermo Fisher Scientific), antiMICA (ab150355, 1:1000 dilution; Abcam), anti- MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; SigmaAldrich), anti- CD9 (13 174S, 1:1000 dilution; CST), antiCD63 (ab59479, 1:1000 dilution; Abcam), anti- CD81 (56 039S, 1:1000 dilution; CST), anti- ERp5 (1:2500; 18 233–1- AP, Proteintech, Rosemont, Illinois, USA) and anti- RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or IgG2B isotype control antibody (IC0041G, R&D systems) and (2) anti- HLA- E (342603; BioLegend, San Diego, California, USA), antiHLA- ABC (555552; BD Biosciences, San Jose, California, USA) or an IgG1 isotype control antibody (555748; BD Biosciences) for 30 min on ice. ..

    Incubation:

    Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells
    Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or IgG2B isotype control antibody (IC0041G, R&D systems) and (2) anti-HLA-E (342603; BioLegend, San Diego, California, USA), anti-HLA-ABC (555552; BD Biosciences, San Jose, California, USA) or an IgG1 isotype control antibody (555748; BD Biosciences) for 30 min on ice. ..

    Article Title: MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells.
    Article Snippet: Total protein lysates from cultured cells were subjected to immunoblot analysis using anti- MUC1- C (HM- 1630- P1ABX, 1:100 dilution; Thermo Fisher Scientific), antiMICA (ab150355, 1:1000 dilution; Abcam), anti- MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; SigmaAldrich), anti- CD9 (13 174S, 1:1000 dilution; CST), antiCD63 (ab59479, 1:1000 dilution; Abcam), anti- CD81 (56 039S, 1:1000 dilution; CST), anti- ERp5 (1:2500; 18 233–1- AP, Proteintech, Rosemont, Illinois, USA) and anti- RAB27A (69 295S, 1:1000 dilution; CST). .. Cells were washed with ice cold phosphate buffered saline (PBS) and incubated with: (1) Alexa Fluor 488 conjugated MICA antibody (FAB1300G, R&D Systems, Minneapolis, Minnesota, USA), Alexa Fluor 488 conjugated MICB antibody (FAB1599G, R&D Systems) or IgG2B isotype control antibody (IC0041G, R&D systems) and (2) anti- HLA- E (342603; BioLegend, San Diego, California, USA), antiHLA- ABC (555552; BD Biosciences, San Jose, California, USA) or an IgG1 isotype control antibody (555748; BD Biosciences) for 30 min on ice. ..



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    Image Search Results


    A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of CD24 staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.

    Journal: bioRxiv

    Article Title: Acute degron-mediated RUNX1 loss reprograms enhancer activity to epigenetically drive epithelial destabilization and initiate cancer hallmarks

    doi: 10.64898/2026.03.26.711344

    Figure Lengend Snippet: A) Chromosomal tracks depicting RUNX1 binding, chromatin contacts, ATAC and H3K27ac signals at chr15-101Mb region encompassing the ALDH1A3 locus that is upregulated upon the loss of RUNX1. B) Flow cytometry histograms of ALDH activity measured by an ALDEred assay at different timepoints following RUNX1 ablation. C) Quantification of ALDH high cells above DEAB-ALDH inhibitory controls indicate the percentage of cells that are ALDH bright in the DMSO baseline versus dTAGV1 treatment. D) Flow cytometry histograms of CD24 staining at different timepoints following RUNX1 ablation. E) Quantification of CD24+ cells above isotype controls indicates the percentage of CD24 high non-BCSCs in DMSO versus dTAGV1 treatment. F) MCF10A-R1F cells initially treated for 24hrs with DMSO or dTAGV1 were then subsequently placed in an anchorage-independent condition demonstrating an increased number of viable cells upon RUNX1 ablation. G) Relative MTS absorbance (450nm) as a measure of cell metabolism/ viability. Values are expressed as a percentage of vehicle control absorbance at multiple 4-hydroxycyclophosphamide (4-HC) dosages at 48hrs.

    Article Snippet: Cells were stained with CD24 (Miltenyi Biotec, 130-108-352) or isotype (Miltenyi Biotec, 130-124-062) antibodies at the manufacturer’s suggested concentration for 20 minutes at 4°C, washed and acquired using a MACSquant YVB.

    Techniques: Binding Assay, Flow Cytometry, Activity Assay, Staining, Control